搜索结果: 1-7 共查到“兽医科学基础学科 Time”相关记录7条 . 查询时间(0.39 秒)
Evaluation of reference genes for gene expression studies in pig muscle tissue by real-time PCR
gene stability reference genes quantitative PCR Sus scrofa age categories of pigs muscle tissue
2015/6/9
The selection of reference genes is essential for gene expression studies when using a real-time quantitative polymerase chain reaction (PCR). Reference gene selection should be performed for each exp...
Comparison of immune parameters in cows with normal and prolonged involution time of uterus
dairy cows periparturient period phagocytes lymphocytes cell-mediated immune functions immu-nosuppression flow cytometry
2015/1/26
Indices of cellular immunity in postpartum Holsteincows with the normal (n = 9) and prolonged (n = 4) uterine involution time were evaluated. Peripheral white blood cells were isolated by lysis from p...
本研究旨在揭示感染JSRV病羊体内检测不到循环抗体的免疫学机理。用地高辛（DIG）标记制备enJSRV-env探针，原位杂交法检测妊娠70 d的绵羊胎儿（免疫器官初步形成期）、妊娠130 d胎儿（即将出生的）和出生7日龄羔羊的胸腺、脾脏、肠淋巴结和肺脏内enJSRV mRNA表达情况。并利用Real-Time PCR法，对enJSRV mRNA在以上组织中的表达进行定量分析。结果表明，在所检测组织...
Time Course Changes in 13C Enrichment of Respiratory CO2 in Chickens Intravenously Single-Injected with [1-13C] Sodium Acetate
oxidation intravenous acetate 13C enrichment chicks
2009/7/31
Time course changes in 13C enrichment of respiratory CO2 in young chickens intravenously injected with [1-13C] sodium acetate were examined to clarify the rapidity of oxidation of intravenous acetate...
Comparative Effects of Intravenously Single-injected D-[U-13C]Glucose and [1-13C]Sodium Acetate on Time Course Changes in 13C Enrichment of Respiratory CO2 in Growing Chickens
growing chicken glucose oxidation acetate oxidation respiratory 13CO2
2009/7/31
This study was carried out to examine which of intravenous glucose or acetate would be rapidly oxidized to CO2 in growing chickens fed a semi-purified diet.
Noninvasive Measurement of Fecal Progesterone Concentration in Toy Poodles by Time Resolved Fluoroimmunoassay（TR-FIA）
Feed deprivation laying hens molting non-feed removal
2009/1/19
American Journal of Animal and Veterinary Sciences 3(1): 43-46 , 2008
Abstract: Progesterone is an important reproductive hormone and measurement of its level by repeated blood samplings is benefici...
利用SYBR Green建立了检测种特异性衣原体的Real-Time PCR方法。本方法应用衣原体种特异性的高度保守特异引物，能够扩增627 bp特异片段；使用定量标准基因组DNA，本方法能准确检测最少250 fg衣原体DNA。Real-Time PCR方法与免疫荧光方法的检测结果表明：检测4种衣原体临床样本，Real-Time PCR敏感性均在96%~98%；特异性均为100%；这两种方法符合率...