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Comparison of two vitrification methods for cryopreservation of porcine embryos
pig embryo transfer
2015/6/1
The aim of this study was to compare two vitrification methods of porcine perihatching blastocysts with regard to the success of transfer of these embryos to the recipients. Expanded, hatching, or hat...
Single fixed-time laparoscopic intrauterine insemination as a tool to obtain low-diversity porcine embryos
laparoscopic insemination embryo diversity pig
2015/4/16
Double fixed-time insemination after ovulation induction is commonly used in pigs to obtain in vivo produced embryos at defined stages of development for downstream biotechnological applications. Howe...
Production of Mutated Porcine Embryos Using Zinc Finger Nucleases and a Reporter-based Cell Enrichment System
Knockout Pig Reporter Based Enrichment Somatic Cell Nuclear Transfer Zinc Finger Nuclease
2016/5/16
To facilitate the construction of genetically-modified pigs, we produced cloned embryos derived from porcine fibroblasts transfected with a pair of engineered zinc finger nuclease (ZFN) plasmids to cr...
Addition of Macromolecules to PZM-3 Culture Medium on the Development and Hatching of In vitro Porcine Embryos
Porcine Embryos Macromolecule Blastocysts Cell Numbe
2016/4/27
In this study, we conducted various experiments in order to develop enhanced cultural conditions for in vitro-produced porcine embryos. All embryos were produced by in vitro maturation (IVM) and ferti...